2-photon FLIM-FRET imaging of acute kidney slices and more art by Nelli Rutkowski

2-photon FLIM-FRET imaging of acute kidney slices

Acute kidney slices from transgenic mice expressing a FRET-based cGMP biosensor are shown: Baseline (left) and after stimulation with natriuretic peptide BNP (right). The raw FLIM data were fitted to a biexponential decay, subjected to image segmentation, and a false-color image was generated. Prolonged lifetime demonstrates increased cGMP synthesis after stimulation.

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